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MARIO
CAPPADOCIA

Étude génétique et moléculaire de la reproduction

Professeur titulaire
Département de sciences biologiques - Université de Montréal

Adjoint au directeur pour le premier cycle
Département de sciences biologiques - Université de Montréal
mario.cappadocia@umontreal.ca
514-343-2104

THÉMATIQUES DE RECHERCHE


L’auto-incompatibilité (AI) est la capacité génétiquement déterminée d’une plante fertile de rejeter son propre pollen. Notre objectif est d’élucider les mécanismes moléculaires de reconnaissance et de signalisation qui entrent en jeu dans ce phénomène. Chez les Solanacées l’AI dépend des éléments d’un locus génétique complexe (locus S) multigénique. Si pollen et pistil expriment des gènes ayant la même forme allèlique au locus S, la croissance du tube pollinique dans le style est bloquée. L’élément du locus-S exprimé dans le pistil est une ribonucléase (S-RNase) dont le rôle est de dégrader l’ARN chez le pollen self, tandis que l’element du locus S exprimé dans le pollen est une protéine F-box. Le rôle de cette F-box reste à définir: normalement, les F-box sont impliquées dans la dégradation des protéines. Un modèle pour l’AI propose que les F-box dégraderaient les S-RNases non-self lors des croisements compatibles. Le mécanisme par lequel une S-RNase non-self serait reconnue et dégradée n’est pas connu. Selon un autre modèle, les S-RNases non-self seraient séquestrées à l’intérieur d’une vacuole. Le mécanisme par lequel une S-RNase self serait reconnue et relâché de la vacuole par une F-box cytoplasmique n’est pas compris.

En collaboration avec les Drs. David Morse et M. Hijri, nous utilisons les techniques de la génétique inverse (transformation des plantes), de la biochimie et de la biologie cellulaire (immunofluorescence visualisée par microscopie confocale) pour déterminer s’il y a dégradation ou séquestration de la S-RNase dans les tubes polliniques compatibles.

Mots-clés: Étude des barrières intra et interspécifiques lors de la fécondation des plantes à fleurs • Aspects génétiques et moléculaires de l’auto-incompatibilité chez Solanum chacoense



ENSEIGNEMENT


Cours BIO-1203: Introduction à la génétique
Cours BIO-3701: Vitroculture des végétaux



  • Cappadocia, A. Nantel et D.P. Matton. (2015). The FRK1 mitogen-activated protein kinase kinase kinase (MAPKKK) from Solanum chacoense is involved in embryo sac and pollen development. Journal of Experimental Botany, 66 (7): 1833-1843.
  • Lafleur E, Kapfer K, Joly V, Liu Y, Tebbji F, Daigle C, Gray-Mitsumune M, Cappadocia M, Nantel A, and D. Ph. Matton (2015). The FRK1 mitogen-activated protein kinase kinase kinase (MAPKKK) from Solanum chacoense is involved in embryo sac and pollen development J. Exp. Bot. (2015) 66 (7): 1833-1843
  • Roy, S. Beauchemin, M. Dagenais-Bellefeuille, S. Letourneau, L. Cappadocia, M. Morse, D. (2014). The Lingulodinium circadian system lacks rhythmic changes in transcript abundance BMC Biol Dec 20;12:107. doi: 10.1186/s12915-014-0107-z.
  • Boivin N., Morse D., and Cappadocia M. (2014). Degradation of S-RNase in compatible pollen tubes of Solanum chacoense visualized by immunogold labeling J Cell Sci. 127, 4123-4127; doi:10.1242/jcs 154823
  • Soulard, J., N. Boivin, D. Morse and M. Cappadocia (2014). eEF1A Is an S-RNase binding factor in self-incompatible Solanum chacoense. PLoS One 9(2): e90206 (7 p.).
  • Soulard, J., X. Qin, N. Boivin, D. Morse and M. Cappadocia (2013). A new dual-specific incompatibility allele revealed by absence of glycosylation in the conserved C2 site of a Solanum chacoense S-Rnase. J. Exp. Bot. 64(7): 1995-2003.
  • Bolin, L., N. Boivin, D. Morse and M. Cappadocia (2012). A time course GFP expression and mRNA stability in pollen tubes following compatible and incompatible pollinations in Solanum chacoense. Sex Plant Reprod. 25: 205-213.
  • Liu, B., N. Boivin, D. Morse and M.Cappadocia (2012). A time course of GFP expression and mRNA stability in pollen tubes following compatible and incompatible pollinations in Solanum chacoense. Sex. Plant Reprod. 25: 205-213.
  • Liu, B., Morse D. and Cappadocia, M. (2009). Compatible Pollinations in Solanum chacoense Decrease Both S-RNase and S-RNase mRNA. PLoS ONE 4(6): e5774: 1-10
  • Liu, B., Morse D. and Cappadocia, M. (2008). Glycosylation of S-RNases may influence pollen rejection thresholds in Solanum chacoense. J Exp. Bot. 59: 545-552
  • Qin, X., Liu, B., Soulard, J., Morse D. and Cappadocia, M. (2006). Style-by-style analysis of two sporadic self-compatible Solanum chacoense lines supports a primary role for S-RNases in determining pollen rejection thresholds. J Exp. Bot. 57: 2001-2013
  • Qin, X., Soulard, J., Laublin, G., Morse, D. and Cappadocia, M. (2005). Molecular analysis of the conserved C4 region of the S11-RNase of Solanum chacoense. Planta 221: 531-537
  • Nassoury, N., Cappadocia, M. and Morse, D. (2003). Plastid ultrastructure defines the protein input pathway in dinoflagellates. J. Cell Science 116, 2867-2874.
  • Luu,D.T., Qin, X., Laublin, G., Yang, Q., Morse, D., and M. Cappadocia. (2001). Rejection of S-heteroallelic pollen by a dual specific S-RNase predicts a multimeric pollen-S. Genetics 159 : 329-335.
  • Qin, X., Luu, D.T., Yang, Q., Maes, O., Matton, D., Morse, D. and M. Cappadocia. (2001). Genotype-dependent differences in S12-RNase expression lead to sporadic self-compatibility in Solanum chacoense Plant Mol Biol 45: 295-305.
  • Luu, D.T., Qin, X., Morse, D. and M. Cappadocia. (2000). S-RNase uptake by compatible pollen tubes in gametophytic self-incompatibility Nature 407: 649-651.
  • Matton, D., Luu, D.T., Morse, D. and M. Cappadocia. (2000). Reply to: Establishing a paradigm for the generation of new S-alleles. Letter to the editor Plant Cell 12: 313-315.
  • Matton, D., Luu, D.T., Qin, X., Laublin, G., O’Brien, M., Maes, O., Morse, D. and M. Cappadocia. (1999). The production of an S-RNase with dual specificity suggests a novel hypothesis for the generation of new S-alleles Plant Cell 11: 2087-2097.
  • M. Cappadocia. and K.S. Ramulu. (1998). Anther culture in potato improvement. In Khurana, S.M. and R. Chandra (eds) Comprehensive Potato Biotechnology. pp.1-30. Malhotra Publ. New Delhi.
  • Birhman R.K. and M. Cappadocia. (1998). DNA fingerprinting in potato. In Kurana: S.M. and R. Chandra (eds) Comprehensive Potato Biotechnology. pp. 73-96. Malhotra Publ. New Delhi.
  • Matton, D.P., Bertrand, C., Laublin, G. and M. Cappadocia. (1998). Molecular aspects of self-incompatibility in tuber-bearing Solanum species. In Khurana, S.M. and R. Chandra (eds) Comprehensive Potato Biotechnology. pp. 97-114. Malhotra Publ. New Delhi.
  • Matton, D, Morse, D. and M. Cappadocia. (1998). Reply to: Are the hypervariable regions of S-RNases sufficient for allele-specific recognition of pollen? Letter to the editor. Plant Cell 10: 316-317.
  • Matton, D., Maës, O., Laublin, G., Xike, Q., Bertrand, C., Morse, D. M. Cappadocia. (1997). Hypervariable domains of self-incompatibility RNases mediate allele-specific pollen recognition. Plant Cell 9: 1757-1766.
  • Luu, D.-T., Heizmann, Ph., Dumas, C. and M. Cappadocia. (1997). Involvement of SLR1 gene in pollen adhesion to stigmatic surface in Brassicaceae. Sex. Plant Reprod. 10: 227-235.
  • Cloutier, S., M. Cappadocia. and B. Landry. (1997). Analysis of RFLP mapping inaccuracy in Brassica napus L. Theor. Appl. Genet. 95: 83-91.
  • Van Sint Jan V., Laublin, G., Birhman, R.K. and M. Cappadocia. (1996). Genetic analysis of leaf explant regenerability in Solanum chacoense Bitt. Plant Cell Tissue Org. Cult. 47: 9-13.
  • Rivard, S., M. Cappadocia. and B. Landry (1996). A comparison of RFLP maps based on anther-derived selfed and hybrid progenies of Solanum chacoense. Genome 39: 611-621.
  • St-Pierre, B., Bertrand, A., Camirand, A., M. Cappadocia.and N. Brisson (1996). Expression of the potato starch phosphorilase gene: evidence of post transcriptional regulation. Plant Mol. Biol. 30: 1087-1098.
  • Birhman, R.K., Rivard, S.R. and M. Cappadocia. (1996). Segregation of RFLP markers In Solanum chacoense Bitt Proc. Second Asia-Pacific Conf. on Agric.Biotech. pp 127-134. Oxford-IBH Publ.
  • Cloutier, S., M. Cappadocia. and Landry, B. (1995). Study of microspore culture responsiveness in oilseed rape (B. napus) by comparative RFLP mapping of an F2 and two microspore derived populations. Theor. Appl. Genet. 91: 841-847.
  • Birhman, K. Laublin, G. and M. Cappadocia. (1994a). Genetic control of in vitro shoot regeneration from leaf explants in Solanum chacoense Bitt. Theor. Appl. Genet. 88: 535-540.
  • Birhman, R.K., Laublin, G. and M. Cappadocia. (1994b). Inheritance of yellow cotyledon seedling mutant in Solanum chacoense Bitt. Journal of Heredity. 85: 241-242.
  • Birhman, K., Rivard, S., and M. Cappadocia. (1994). Origin of anther-culture derived plants in Solanum chacoense Bitt. determined by RFLP analysis. Hort. Science. 29: 206-208.
  • Saba El Leil, M., Rivard, S., Morse, D. and M. Cappadocia. (1994). The S11 and S13 self-incompatibility alleles in Solanum chacoense are remarkably similar. Plant Mol. Biol. 24: 571-583.
  • Després, C., M. Saba El Leil, S. Rivard, D. Morse and M. Cappadocia. (1994). Molecular cloning of two Solanum chacoense S-alleles and a hypothesis concerning their evolution. Sex. Plant Reprod. 76: 169-176.
  • Rivard, S., Saba El Leil, M., Landry, B. and M. Cappadocia. (1994). RFLP analyses and segregation of molecular markers in plants produced by in vitro anther culture, selfing and reciprocal crosses of two lines of self-incompatible Solanum chacoense Bitt. Genome. 37: 775-783
  • Birhman, K., Brault, M. and M. Cappadocia. (1994). Genetic analysis of in vitro growth and microtuber production in Solanum chacoense J. Indian Pot. Assoc. 21: 195-200.
  • M. Cappadocia. M., Heizmann, P. and C. Dumas (1993). Application of tissue printing technique to self-incompatibility studies in B. oleracea and S. chacoense Plant Mol. Biol. 23: 1079-1085.
  • Véronneau, H., G. Lavoie and M. Cappadocia. (1992). Genetic analysis of anther and leaf disc culture in two clones of S. chacoense Bitt. and their reciprocal hybrids. Plant Cell Tissue and Organ Culture. 30: 199-209.
  • Laublin, G. and M. Cappadocia. (1992). In vitro plant regeneration from cultured ovaries of some rhizomatous irise. Botanica Acta. 105: 319-322.
  • Vieth, J., Laublin, G., Morisset, C. and M. Cappadocia. (1992). Multiplication in vitro de quelques Iris à partir de racines: aspect histologique de l’embryogenèse somatique. Can. J. Bot. 70: 1809-1814.
  • Jean, M. and M. Cappadocia. (1992). Effects of some growth regulators on in vitro tuberization in Dioscorea alata cv. Brazo fuerte and D. abyssinica Hoch. Plant Cell Reports. 11: 34-38.
  • Lauzer, D., Laublin, G. Vincent, G. and M. Cappadocia. (1992). In vitro propagation and cytology of wild yams, Dioscorea abyssinica Hoch. and D. mangenotiana Miege. Plant Cell Tissue and Organ Culture. 28: 215-223.

Institut de recherche en biologie vegetale.

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